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Dietary Bacillus subtilis C-3102 spores influence intestinal (excreta) populations of Lactobacilli, Clostridium perfringens, Enterobacteriacae (coliforms), and Salmonella, and live performance of broiler chickens.

M. Kato1, N. Otomo1, K. Nishimura2, Y. Tadano3, T. Marubashi3, H. Miyazaki3,
K. Maruta3 and D. M. Hooge4
1Calpis USA, Inc., Schaumburg, IL, 2Quality Tech. Int’l, Inc., Elgin, IL,3 Calpis Co. Ltd, Tokyo, Japan, and 4Hooge Consulting Service, Inc., Eagle Mountain, UT.

Abstract

Intestinal Lactobacilli (Lac) counts and Lactobacilli/Total Anaerobes (Lac/TA%; 50% or more optimal) are important in relationship to broiler performance and pathogen loads. CALSPORIN® (Calpis Co. Ltd, Tokyo, Japan) has Bacillus subtilis C-3102 (Bs) aerobic spores which vegetate in digesta and consume oxygen causing proliferation of native colonizing, facultatively anaerobic Lactobacilli to help improve bird performance and reduce pathogen loads. In Exp. 1 in southern Japan, Bs was added to all broiler feeds (0 or 3x105 CFU/g), and excreta collected at 2 ages. At 14 d, in the Bs flock Lac increased (P<0.05) and Salmonella were not detected vs 4.97 Log10 CFU/g excreta in the negative control flock. At 49 d, Clostridium perfringens (3.22 vs 2.66 Log10 CFU/g excreta; P<0.05) and Salmonella (4.07 vs 3.31 Log10CFU/g excreta, P<0.01; 20/20 vs 10/20, P<0.05) were reduced in the Bs flock. Two trials were conducted at a US broiler company to compare Bs fed flocks vs previous antibiotic growth promoter (AGP; for necrotic enteritis prevention) fed flocks. Excreta was collected at 35 d. In Exp. 2, Lac (P<0.001) and Lac/TA% (22.9% vs 52.2%; P<0.05) were higher and Enterobacteriacae (coliforms) lower (P<0.01) in Bs birds. Livability was higher and farm condemnations lower in 7 Bs flocks vs 4 previous AGP flocks (P<0.01), and caloric conversion was lower in 7 Bs flocks vs 902 simultaneous AGP flocks (P=0.014). In Exp. 3a, Lac (P<0.001) and Lac/TA% (21.2% vs 58.5%; P<0.01) were higher in Bs birds. In Exp. 3b (same control as Exp. 3a), Lac (P<0.001) and Lac/TA% (21.2% vs 54.8 and 59.5% P<0.001) were higher and Clostridium perfringens lower (P<0.05) in Bs birds. The Bs C-3102 flocks had higher intestinal (excreta) Lac or Lac/TA%, lower pathogen counts, and/or improved performance, compared to previous (or simultaneous) AGP flocks.

Key Words:Bacillus subtilis C-3102, Lactobacilli, pathogens